Four-dimensional multi-site photolysis of caged neurotransmitters
نویسندگان
چکیده
منابع مشابه
Four-dimensional multi-site photolysis of caged neurotransmitters
Neurons receive thousands of synaptic inputs that are distributed in space and time. The systematic study of how neurons process these inputs requires a technique to stimulate multiple yet highly targeted points of interest along the neuron's dendritic tree. Three-dimensional multi-focal patterns produced via holographic projection combined with two-photon photolysis of caged compounds can prov...
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Photolabile ‘caged’ compounds are biologically inert precursors of active molecules, which when irradiated with a pulse of light free the active species at its site of action (see Fig. 1). Speed is the principal advantage offered by these probes; photochemical reactions can be very fast, with release of the active species often complete within a millisecond. The method can thus be used to study...
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Caged compounds are biologically active molecules that have been rendered inert by the introduction of a photolabile protecting (caging) group to an important functional group. The intracellular photolysis of the caging group to reveal the active compound provides a powerful method for the intracellular release of biologically important molecules with exquisite temporal and spatial control. Thi...
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To understand the precise microarchitecture of the cortical circuitry, it is crucial to know the distribution of synaptic connections and their synaptic strengths at the level of a single cell, rather than a group of cells. Here, we describe a new application of two-photon photolysis of caged glutamate that enabled us to induce an action potential in only a small number (about five) of pyramida...
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ژورنال
عنوان ژورنال: Frontiers in Cellular Neuroscience
سال: 2013
ISSN: 1662-5102
DOI: 10.3389/fncel.2013.00231